hen with a weak solution of
potassic hydrate, to cause the spores and other structures to assume
proper plumpness. A little glycerine may be added or run under the cover
if it is desired to preserve the material for further or prolonged
study. For permanent mounting nothing in most cases is better than
glycerine jelly. As a preparation, the material should lie for some time
in Haentsch's fluid,[14] opportunity being given for evaporation of the
alcohol and water. When the material shows the proper clearness and
fulness, it may be mounted in jelly in the usual way. Kaiser's formula
gives beautiful results. After mounting, the preparation should be
sealed with some good cement, as Hollis's glue.

FOOTNOTES:

[4] DeBary, _Morphology and Biology of the Fungi,_ p. 428.

[5] See, however, _Ceratiomyxa_, p. 18, following.

[6] Harper in _Botanical Gazette_, Vol. XXX., p. 219.

[7] The following germination periods are furnished by
Dr. Constantineanu (_Inaugural Dissertation ueber die
Entwickelungsbedingungen der Myxomyceten_; Halle, 1907).

_Reticularia lycoperdon_ 30 to 60 min.
_Fuligo ovata_ 30 to 90 min.
_Stemonitis splendens_ 5 to 6 hrs.
_Perichaena depressa_ 5 to 8 hrs.
_Amaurochaete atra_ 6 to 10 hrs.
_Arcyria incarnata_ 8 to 10 hrs.
_Lycogala epidendrum_ to 60 hrs.
_Physarum didermoides_ 1 to 10 da.
_Dictydium cancellatum_ 1 to 20 da.

These records are for sowings in drop cultures, in distilled water, kept
at temperature of 65 deg.-70 deg. F. (18 deg.-20 deg. C.).

Our own experiments have been made both with distilled water and
tap-water with the advantage in favor of the latter. _Dictydium
cancellatum_ germinates in tap-water at temperature 70 deg.-80 deg. F. in 12-15
hours fresh from the field. _Fuligo ovata_ spores were all swarming in
about one hour at the same temperature. Jahn (_Myxomycetenstudien; Ber.
der Deutschen Bot. Ges._ Bd. XXIII., p. 495) finds that the germination
in some cases as _Stemonitis_ species, is hastened by wetting, then
drying, then wetting again.

Pinoy thinks microbes aid in germination (_Bull. Soc. Myc. de France_ T.
XVIII.).

[8] The plasmodium in this case chances to be red, scarlet, et