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d renders so far persistent the delicate sporangium. For more exact description the reader is again referred to the specific delineations which follow. The transition from phase to phase requires, as intimated, no great length of time. _Tilmadoche polycephala_ completed the transition from vegetative to fruiting phase in less than twelve hours. The germination of the spores ensues closely upon their dispersal or maturity and is unique in many respects.[7] The wall of the spore is ruptured and the protoplasmic content escapes as a zoospore indistinguishable so far from an amoeba, or from the zoospore of our chytridiaceous fungi. This amoeboid zoospore is without cell-wall, changes its outline, and moves slowly by creeping or flowing from point to point. At this stage many of the spores assume each a flagellate cilium, and so acquire power of more rapid locomotion. The zoospores, whether ciliate or not, thus enjoy independent existence and are capable of continuing such existence for some time, assimilating, growing, and even reproducing themselves by simple fission, over and over again. This takes place, of course, only in the presence of suitable nutrient media. Nevertheless the spores of many species germinate quickly simply in water, and a drop suspended in the form of the ordinary drop-culture on a cover-glass affords ample opportunity. In the course of time, usually not more than two or three days, the swarm spores cease their activity, lose their cilia, and come to rest, exhibiting at most nothing more than the slow amoeboid movement already referred to. In the course of two or three days more, in favorable cases, the little spores begin to assemble and flow together; at first into small aggregations, then larger, until at length all have blended in one creeping protoplasmic mass to form thus once again the plasmodium, or plasmodial phase with which the round began. Small plasmodia may generally be thus obtained artificially from drop-cultures. Such, however, in the experience of the writer, are with difficulty kept alive. Hay infusions, infusions of rotten wood, etc., may sometimes for a time give excellent results. The spores of _Didymium crustaceum_ were sown upon a heap of leaves in autumn. An abundant display of the same species followed in the next June; but, of course, the intervening phases were not observed. The most satisfactory studies are obtained by plasmodia carefully brought in directly from t
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