te of these appearances.
They first appear as point-like granules in the protoplasm,
bearing no relation to the cell nucleus; they gradually
increase, and acquire a considerable circumference. When they
have attained about the size of the cell nucleus, they, or
rather their contents, appear to break through the protoplasmic
membrane and to leave the cell.

The number of the vacuole containing cells is 15-20% of the
colourless blood corpuscles.

([beta]) Typical lymphocytes.

Their appearance completely corresponds with that of human
lymphocytes as described above. They make up 30-35% of the
total number of leucocytes.

Now Kurloff in the course of extremely careful and laborious
researches, estimated the total number of leucocytes, and then
from the percentage numbers, the total quantity of
pseudoeosinophil, neutrophil, eosinophil, vacuole containing
cells, and lymphocytes, and could thus demonstrate that in
uncomplicated cases of removal of the spleen, where
inflammatory processes, accompanied by an increase of the
polynuclear neutrophil corpuscles, were avoided, a =gradual
increase of the lymphocytes= alone in course of time results.
This may be a two- or threefold increase, whereas the numbers
of all other elements remain unchanged.

Kurloff obtained his figures as follows: first he estimated the relative
proportion of the different kinds of white blood corpuscles one to
another in a large number of cells (500 to 1000). A count of this kind
however gives no evidence as to whether one or other kind of cell is
absolutely increased or diminished. A fall in the percentage of the
lymph cells may be brought about by two quite different factors: (1) by
a diminished production of lymphocytes, (2) by an increased influx of
polynuclear forms, which naturally lowers the relative count of the
lymphocytes. It was therefore necessary to obtain a method which would
show alterations in the absolute number of the individual forms of
leucocytes. Kurloff used for this purpose the "comparative field"; that
is, he counted by the aid of a moveable stage the different forms which
lay on a definite area (22 sq. mm.) of the dried blood preparation. This
procedure gave very exact results, as only faultlessly prepared, and
regularly spread preparations were used. The following figures (from
Exp. II.) illustrate the method