ting that pupae of
two species had been collected. Figure 187 shows the metaphase of first
spermatocyte mitosis with the unequal pair in metakinesis. Figures 188
and 189 are first spermatocyte equatorial plates of the two species,
containing 10 and 11 chromosomes respectively. Figure 190 is a first
spermatocyte spindle in anaphase, showing the unequal pair behind the
other chromosomes. Figure 191 is an equatorial plate from a second
spermatocyte, showing the small chromosome. In figure 192 are shown
several of the bivalent chromosomes, including the unsymmetrical pair,
from nuclear prophases of the first division, all from the same cyst.

_Adalia bipunctata_ (family Coccinellidae), the common lady beetle, has a
very conspicuous pair of unequal heterochromosomes, as may be seen in
figures 193-197 (plate XIII). This would seem to be a favorable form for
determining the chromosome conditions in somatic cells, but no clear
equatorial plates were found in either larvae or pupae.

In _Cicindela primeriana_ (family Cicindelidae) there are 18 chromosomes
in the spermatogonium (fig. 198), one being small. The heterochromosome
group is blended into a vacuolated sphere in growth stages (figs. 199,
200). In the metaphase of the first division it is trilobed, or
tripartite (fig. 201), and in metakinesis, a small spherical chromosome
separates from a much larger V-shaped one (fig. 202). Equatorial plates
of first and second spermatocytes are shown in figures 203 and 204.
Whole cysts of giant first spermatocytes were found both in growth
stages (fig. 205) and prophases (fig. 206). Here the heterochromosome
group is plainly double (fig. 205), and the conditions observed must
have been due to the failure of a spermatogonial mitosis to complete
itself.

Several of the Carabidae have been studied, and the material, though not
especially favorable, is interesting in that some members of the family
have an unequal pair of heterochromosomes, others an odd one. _Chlaenius
aestivus_ (figs. 207-212), _Chlaenius pennsylvanicus_ (figs. 213-215), and
_Galerita bicolor_ (fig. 216) have the unequal pair, while _Anomoglossus
emarginatus_ (figs. 217-223) has an odd heterochromosome (_x_), which
behaves exactly like the larger heterochromosome in other carabs.

In the Elateridae and Lampyridae we also have examples of the second type
with the odd chromosome. Two Elaters, species not determined (figs.
224-229 and 230-235), have each 19 chromosomes in