. All of the spermatids (figs. 266-268) contain, in the
early stages of development, a body (_n_) which stains like chromatin,
but increases in size from a small granule in the telophase (figs. 264,
265) to the large dense body (_n_) seen in figure 267. This is probably
homologous with the chromatin nucleolus described for the spermatids of
the Coleoptera. In addition to this, in one-half of the spermatid nuclei
there is a condensed mass of chromatin which is evidently the derivative
of the odd chromosome of the spermatogonia and spermatocytes (figs. 267
and 268, _x_). In common with the spermatids of other Hemiptera these
show two masses of archoplasm, the larger of which forms the sheath
(_s_) of the axial fiber of the tail, and the smaller the acrosome
(_a_). The axial fiber grows out directly from the centrosome, on either
side of which there is a dense band forming the lateral boundary of the
middle piece. It will be seen that the odd chromosome of Aphrophora is
in its behavior precisely like the typical Orthopteran "accessory" of
McClung, and similar to the odd chromosome of the Coleoptera.

In various parts of the young male larvae dividing cells were found and
the number 23 determined (fig. 269). Turning now to the female larvae to
determine the somatic number, the oogonia proved to be more favorable
for counting. Twenty-four chromosomes were present in equatorial plates
of oogonial mitoses (fig. 270), thus confirming Wilson's results for the
_Anasa_ group of the Hemiptera heteroptera.

In examining sections of female larvae stained with
safranin-gentian-violet, I was surprised to see a very marked polarized
or bouquet stage and to find among the loops something resembling the
odd chromosome of the growing spermatocytes. It was difficult to get a
clear view of this body as it lay within the loops. In one section of a
slightly earlier stage before synapsis, there were found two pairs of
chromosomes (fig. 271, _x_{1}_, _x_{2}_, and _m_{1}_, _m_{2}_) which
were stained with safranin in contrast with the violet spireme. These
two pairs I interpret as being (1) the homologues of the pair of
_m_-chromosomes, which remain condensed during the growth stage of the
spermatocytes, and (2) a pair of heterochromosomes corresponding to the
odd chromosome of the male. Various combinations of these
heterochromosomes are shown in figures 272-277. Figures 278 and 279 were
taken from mercuro-nitric material stained with iron-haema