nnsylvanicus._
{ 3. _Galerita bicolor._

{ 1. _Blepharida rhois._
{ 2. _Chelymorpha argus._
{ 3. _Coptocycla aurichalcea._
III. Chrysomelidae { 4. _Coptocycla guttata._
{ 5. _Doryphora decemlineata._
{ 6. _Odontota dorsalis._
{ 7. _Trirhabda virgata._
{ 8. _Trirhabda canadense._

IV. Cicindelidae _Cicindela primeriana._

V. Coccinellidae { _Adalia bipunctata._
{ _Epilachna borealis._

VI. Scarabaeidae _Euphoria inda._

VII. Silphidae _Silpha americana._

VIII. Tenebrionidae _Tenebrio molitor._

(2) An odd chromosome, which behaves during the growth stage of the
first spermatocytes like the "accessory" of the Orthoptera, has been
found in 4 species of Coleoptera,[A] belonging to 3 families:

FAMILY. SPECIES.

I. Carabidae _Anomoglossus emarginatus._

II. Elateridae Two Elaters; species not determined.

III. Lampyridae _Ellychnia corrusca._

(3) In most of the species of Coleoptera examined, the unequal pair or
the odd chromosome remains condensed during the growth period of the
first spermatocyte, like the "accessory" of the Orthoptera and the
various heterochromosomes of the Hemiptera.

(4) Several of these species of Coleoptera have a synizesis stage in
which the spermatogonial number of short loops is massed at one side of
the nucleus. This is followed by a synapsis stage in which the loops
straighten and unite in pairs, forming longer loops which soon spread
out in the nuclear space, and, with the exception of the
heterochromosomes, unite to form a continuous spireme.

(5) In several of the species of Coleoptera and in Aphrophora, it has
been shown that a body staining like chromatin develops in the
spermatids, increasing in size for a time, then breaking up into
granules and disappearing. This body evidently has no relation to the
heterochromosomes, as it is the same for all of the spermatids. Its
staining qualities suggest that it may be material derived from the
chromosomes. It is finally dissolved in the karyolymph.

(6) In iron-haematoxylin preparations the heterochromosomes of the
Coleoptera vary greatly in their staining properties during mitosis. In
some species they stain exactly like the ordinary chromosomes, in others
the larger one of the unequal