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fusion of the substance of the sperm filament with that of the egg in such a way that there is a distinct and intimate fusion of the nucleus of the sperm filament with the nucleus of the egg-cell. The recent discovery of M. Bataillon is this, viz. that you can make the frog's egg develop in a perfectly regular way and become a tadpole and then a young frog without the admission to it of a sperm-filament or of any substance derived from the male frog. All you have to do--and the operation, though it sounds easy and simple, is an exceedingly delicate and difficult one--is to prick with a fine needle the surface of the little black egg-sphere (not merely of the jelly surrounding it) when it is shed by the female frog into perfectly pure water free from sperms or anything of the sort. The slight artificial puncture acts as does the natural puncture by the swimming sperm-filament, and is sufficient! The egg proceeds to develop quite regularly. There is no fusion of the nucleus of the egg-cell with any matter from the outside; no paternal "material" is introduced, but the nucleus of the egg-cell divides just as though there had been! The whole progeny of cells, successively formed, are the pure offspring of the maternal egg-cell and its nucleus. The tadpoles and young frogs so produced are examples of what is called "parthenogenesis"--that is to say, virginal reproduction--reproduction without fertilisation by material derived from a male parent! The needle, which gives off no material, but simply makes a tiny break in the surface of the egg, does all that is necessary! To those not acquainted with all that has been ascertained as to the reproduction of lower animals such as insects, crustaceans, and worms, this discovery will appear more astonishing than it really is. We know of many lower animals in which the egg-cells produced by the females do regularly and naturally develop without the intervention of a male and without fertilisation. In an earlier volume[7] of this "Easy Chair Series" I wrote of this curious subject, and described the virgin reproduction or parthenogenesis of the hop-louse and other plant lice, of some moths, of some fresh-water shrimps, and of the queen bee (who produces only drones by eggs which are not fertilised). But I had to point out then that no case was known of "parthenogenesis"--that is to say, reproduction by unfertilised eggs--among the whole series of vertebrate animals, the fishes, amp
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