also have an interest, though not of as
great importance. In order to obtain the pulse rate, we attach a Bowles
stethoscope over the apex beat of the heart and hold it in place with a
light canvas harness. Through a long transmission-tube passing through
an air-tight closure in the walls of the calorimeter it is possible to
count the beats of the heart without difficulty. The respiration rate is
determined by attaching a Fitz pneumograph about the trunk, midway
between the nipples and the umbilicus. The excursions of the tambour
pointer as recorded on the smoked paper of the kymograph give a true
picture of the respiration rate.

Of still more importance, however, is the fact that the expansion and
contraction of the pneumograph afford an excellent means for noting the
minor muscular activity of a subject, otherwise considered at complete
rest. The slightest movement of the arm or the contraction or relaxation
of any of the muscles of the body-trunk results in a movement of the
tambour quite distinct from the respiratory movements of the thorax or
abdomen. These movements form a very true picture of the muscular
movements of the subject, and these graphic records have been of very
great value in interpreting the results of many of the experiments.

ROUTINE OF AN EXPERIMENT WITH MAN.

In the numerous previously published reports which describe the
construction of and experiments with the respiration calorimeter, but
little attention has been devoted to a statement of the routine. Since,
with the increasing interest in this form of apparatus and the possible
construction of others of similar form, a detailed description of the
routine would be of advantage, it is here included.

PREPARATION OF SUBJECT.

Prior to an experiment, the subject is usually given either a stipulated
diet for a period of time varying with the nature of the experiment or,
as in the case of some experiments, he is required to go without food
for at least 12 hours preceding. Occasionally it has been deemed
advisable to administer a cup of black coffee without sugar or cream,
and by this means we have succeeded in studying the early stages of
starvation without making it too uncomfortable for the subject. The
stimulating effect of the small amount of black coffee on metabolism is
hardly noticeable and for most experiments it does not introduce any
error.

The urine is collected usually for 24 hours before, in either 6 or 12
hour periods. Du