t-fixation test may be employed.
The mallein test is made by injecting mallein (a sterilized extract from
a culture of glanders bacilli) beneath the skin. If the horse has
glanders there results a febrile reaction and a swelling at the point of
injection. If the horse does not have glanders the mallein has no effect
or, at most, it produces a slight swelling only at the point of
injection. The inoculation test consists in the inoculation of a
susceptible animal (usually a guinea pig) with some of the suspected
discharge from the nose or a farcy ulcer. If the material is properly
used, and if it contains bacilli of glanders, the experimental animal
will develop the disease.

The eye test is now universally accepted as a very satisfactory means of
diagnosing glanders. This consists in dropping into an eye of a
suspected animal a specially prepared solution of mallein, as a result
of which in an infected animal the inflammation develops in the eye,
resulting in a discharge which varies in intensity from a mucopurulent
character to a thick, sticky pus. The eyelids may also swell and many
times become glued together. The reaction usually appears in from 8 to
20 hours after the introduction of the mallein.

Neither of these tests should be put into use except by a competent
veterinarian. The complement-fixation test is a highly specialized
laboratory test and can be carried out only by one versed in laboratory
technique. (See Bureau of Animal Industry Bulletin 136.)

The post-mortem examination of the lungs shows that the pneumonia of
glanders is a lobular, V-shaped pneumonia scattered throughout the lungs
and caused by the specific inflammatory process taking place at the
divergence of the smaller air tubes of the lungs. In some cases of acute
glanders the formation of nodules may so irritate the mucous membrane of
the respiratory tract and cause such a profuse discharge of mucopurulent
or purulent matter that the specific character of the original discharge
is entirely masked. In this case, too, for a few days the submaxillary
space may so swell as to resemble the edematous, inflamed glands of
strangles, equine variola, or laryngitis. This condition is especially
liable to be marked in an acute outbreak of glanders in a drove of
mules.

Cases of chronic farcy and glanders, if not destroyed, may live in a
depraved condition until the animal dies from general emaciation and
anemia, but in the majority of cases, from some