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chemically; the bark and wood of the root and the base and blades of the leaves. Fixed oils were separated from each part. These were not identical; two were fluid at ordinary temperature, and two were solid. Their melting and solidifying points were not the same. This difference in the physical character and chemical reaction of these fixed oils may be due to the presence of free fatty acid and glycerides in varying proportions in the four parts of the plants. It is of interest to note that, in the subterranean part of the _Yucca_, the oil extracted from the bark is solid at the ordinary temperature; from the wood it was of a less solid consistency; while the yellow base of the leaf contained an oil quite soft, and in the green leaf the oil is almost fluid. Two new resins were extracted from the yellow and green parts of the leaf. It was proposed to name them _yuccal_ and _pyrophaeal_ An examination of the contents of each extract showed a different quantitative and qualitative result. Saponin was found in all parts of the plant. Many of the above facts have been collected from the investigations of others. I have introduced these statements, selected from a mass of material, as evidences in favor of the view stated at the beginning of this paper.[41] My own study has been directed toward the discovery of saponin in those plants where it was presumably to be found. The practical use of this theory in plant analysis will lead the chemists at once to a search for those compounds which morphology shows are probably present. I have discovered saponin in all parts of the _Yucca angustifolia_, in the _Y. filimentosa_ and _Y. gloriosa_, in several species of agavae, and in plants belonging to the leguminosae family. The list[42] of plants in which saponin has been discovered is given in the note. All these plants are contained in the middle plane of Heckel's scheme. No plants containing saponin have been found among apetalous groups. No plants have been found containing saponin among the lower monocotyledons. The plane of saponin passes from the liliaceae and allied groups to the rosales and higher dicotyledons. Saponin belongs to a class of substances called glucosides. Under the action of dilute acids, it is split up into two substances, glucose and sopogenin. The chemical nature of this substance is not thoroughly understood. The commercial[43] product is probably a mixture of several substances. This
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