ulation, with a wad of cotton-wool, and lather freely by the aid of
warm water; shave carefully and thoroughly; or apply the depilatory
powder.

3. Wash the denuded area of skin thoroughly with 2 per cent. lysol
solution.

4. Wash off the lysol with ether and allow the latter to evaporate.

5. Make numerous short, parallel, superficial incisions with the point
of a sterile scalpel.

6. When the oozing from the incisions has ceased, rub the inoculum into
the scarifications by means of the flat of a scalpel blade, or a sterile
platinum spatula.

7. Cover the inoculated area with a pad of sterile gauze secured _in
situ_ by strips of adhesive plaster or by sealing down the edges of the
gauze with collodion.

8. Release the animal, place it in its cage, and affix a label upon
which is written:

(a) Distinctive name or number of the animal.
(b) Its weight.
(c) Particulars as to source and dose of inoculum.
(d) Date of inoculation.

~2. Subcutaneous Inoculation.~--

(a) _Fluid Inoculum._--(_Anaesthetic, none._)

Steps 1-4. As for cutaneous inoculation.

5. Pinch up a fold of skin between the forefinger and thumb of the left
hand; take the charged hypodermic syringe in the right hand, enter the
needle into a ridge of skin raised by the left finger and thumb, and
push it steadily onward until about 2 cm. of the needle are lying in the
subcutaneous tissue. Now release the grasp of the left hand and slowly
inject the fluid contained in the syringe.

6. Withdraw the needle, and at the same moment close the puncture with a
wad of cotton wool, to prevent the escape of any of the inoculum. The
injected fluid, unless large in amount, will be absorbed within a very
short time.

7. Label, etc.

(b) _Solid Inoculum.--(Anaesthetic, none; or Ethyl chloride spray.)_

Steps 1-4. As for cutaneous inoculation.

5. Raise a small fold of skin in a pair of forceps, and make a small
incision through the skin with a pair of sharp-pointed scissors or with
the point of a scalpel.

6. Insert a probe through the opening and push it steadily onward in the
subcutaneous tissue, and by lateral movements separate the skin from the
underlying muscles to form a funnel-shaped pocket with its apex toward
the point of entrance.

7. By means of a pair of fine-pointed forceps introduce a small piece of
the inoculum into this pocket and deposit it as far as possible from the
point of entrance.

[Illustration: FIG. 181.--Gl