sical methods~, (e) by ~inoculation methods~, and
the results of these examinations duly recorded.

It must be stated definitely that no micro-organism can be identified by
any _one_ character or property, whether microscopical, biological or
chemical, but that on the contrary its entire life history must be
carefully studied and then its identity established from a consideration
of the sum total of these observations.

In order to give to the recorded results their maximum value it is
essential that they should be exact and systematic, therefore some such
scheme as the following should be adhered to; and especially is this
necessary in describing an organism not previously isolated and studied.

SCHEME OF STUDY.

Designation:

Originally isolated by (_observer's name_) in (_date_), from (_source of
organism_).

~1. Cultural Characters.~--(_Vide_ Macroscopical Examination
of Cultivation, page 261.)

Gelatine plates, }
Gelatine streak, } at 20 deg. C.
Gelatine stab, }
Gelatine shake, }

Agar plates, }
Agar streak or smear, }
Agar stab, }
Inspissated blood-serum, } at 20 deg. C. and 37 deg. C.
Bouillon, }
Litmus milk, }
Potato, }

Special media for the purpose of demonstrating
characteristic appearances.

~2. Morphology~.--(_Vide_ Microscopical Examination of
Cultivations, page 272.)

Vegetative forms:
Shape.
Size.
Motility.
Flagella (if present).
Capsule (if present).
Involution forms.
Pleomorphism (if observed).
Sporing forms (if observed). Of which class?
Staining reactions.

~3. Chemical Products of Growth.~--(_Vide_ Chemical
Examination of Cultivations, page 276.)

Chromogenesis.
Photogenesis.
Enzyme formation.
Fermentation of carbohydrates:
Acid formation.
Alkali formation.
Indol formation.
Phenol formation.
Reducing and oxidising substances.
Gas formation.

~4. Biology.~--(_Vide_ Physical Examination of Cultures, page
295.)

Atmosphere.
Temperature.

Reaction of nutrient media.
Resistance to lethal agents:
Physical:
Desiccation.
Light.
Colours.
Chemical germicides.
Vitality.

~5. Pathogenicity:~

Susceptible animals, subsequently arranged in