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the process of reproduction the cell protoplasm may be extruded, leaving the empty tube unaltered in shape. [Illustration: FIG. 85.--Dragrammatic sketch of composite bacterium to illustrate details of anatomical structure.] [Illustration: FIG. 86.--Plasmolysis.] 3. _Cell Contents._--Protoplasm (mycoprotein) contains a high percentage of nitrogen, but is said to differ from proteid in that it is not precipitated by C_{2}H_{6}O. It is usually homogeneous in appearance--sometimes granular--and may contain oil globules or sap vacuoles (Fig. 85, d), chromatin granules, and even sulphur granules. Sap vacuoles must be distinguished from spores, on the one hand, and the vacuolated appearance due to plasmolysis, on the other. The cell contents may sometimes be differentiated into a parietal layer, and a central body (e. g., beggiotoa) when stained by haematoxylin. 4. _Nucleus._--This structure has not been conclusively proved to exist, but in some bacteria chromatin particles have been observed near the centre of the bacterial cell and denser masses of protoplasm situated at the poles which exhibit a more marked affinity than the rest of the cell protoplasm for aniline dyes. These latter are termed polar granules or _Polkoerner_ (Fig. 85, e). Occasionally these aggregations of protoplasm alter the colour of the dye they take up. They are then known as metachromatic bodies or _Ernstschen Koerner_ (e. g., B. diphtheriae). 5. _Flagella_ (Organs of Locomotion, Fig. 85, a).--These are gelatinous elongations of the cell protoplasm (or more probably of the capsule), occurring either at one pole, at both poles, or scattered around the entire periphery. Flagella are not pseudopodia. The possession of flagella was at one time suggested as a basis for a system of classification, when the following types of ciliation were differentiated (Fig. 87): [Illustration: FIG. 87.--Types of ciliation.] 1. Polar: (a) _Monotrichous_ (a single flagellum situated at one pole; e. g., B. pyocyaneus). (b) _Amphitrichous_ (a single flagellum at each pole; e. g., Spirillum volutans). (c) _Lophotrichous_ (a tuft or bunch of flagella situated at each pole; e. g., B. cyanogenus). 2. Diffuse: _Peritrichous_ (flagella scattered around the entire periphery e. g., B. typhosus). ~PHYSIOLOGY.~ ~Reproduction.~--_Active Stage._--Vegetative, i. e., by the division of cells, or "fission." 1. The cell becomes elongated and the protoplasm
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