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that the green cheese, fresh from the press, would contain practically the same kind of bacteria that are in the milk, but a study of cheese shows a peculiar change in the character of the flora. In the first place, fresh cottage cheese, made by the coagulation of the casein through the action of acid, has a more diversified flora than cheese made with rennet, for the reason, as given by Lafar,[186] that the fermentative process is farther advanced. When different varieties of cheese are made from milk in the same locality, the germ content of even the ripened product has a marked similarity, as is illustrated by Adametz's work[187] on Emmenthaler or Swiss hard cheese, and Schweitzer Hauskaese, a soft variety. Of the nine species of bacilli and cocci found in mature Emmenthaler, eight of them were also present in ripened Hauskaese. Different investigators have studied the bacterial flora of various kinds of cheese, but as yet little comparative systematic work has been done. Freudenreich[188] has determined the character and number of bacteria in Emmenthaler cheese, and Russell[189] the same for cheddar cheese. The same general law has also been noted in Canadian[190] and English[191] cheese. At first a marked decrease in numbers is usually noted, lasting for a day or two. This is followed by an enormous increase, caused by the rapid growth of the lactic-acid type. The development may reach scores of millions and often over a hundred million organisms per gram. Synchronous with this increase, the peptonizing and gas-producing bacteria gradually disappear. This rapid development, which lasts only for a few weeks, is followed by a general decline. In the ripening of cheese a question arises as to whether the process goes on throughout the entire mass of cheese, or whether it is more active at or near the surface. In the case of many of the soft cheese, such as Brie and limburger, bacterial and mold development is exceedingly active on the exterior, and the enzyms secreted by these organisms diffuse toward the interior. That such a condition occurs in the hard type of cheese made with rennet is extremely improbable. Most observers agree that in this type of cheese the ripening progresses throughout the entire mass, although Adametz opposes this view and considers that in Emmenthaler cheese the development of the specific aroma-producing organism occurs in the superficial layers. Jensen has shown, however, that the g
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