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g have to be in keeping with the sex that will proceed from it. And here is a much more puzzling condition: the sex of that egg, whose advent is predestined, has to correspond with the space which the mother happens to have found for a cell. There is therefore no room for hesitation, strange though the statement may appear: the egg, as it descends from its ovarian tube, has no determined sex. It is perhaps during the few hours of its rapid development at the base of its ovarian sheath, it is perhaps on its passage through the oviduct that it receives, at the mother's pleasure, the final impress that will produce, to match the cradle which it has to fill, either a female or a male. PERMUTATIONS OF SEX. Thereupon the following question presents itself. Let us admit that, when the normal conditions remain, a laying would have yielded m females and n males. Then, if my conclusions are correct, it must be in the mother's power, when the conditions are different, to take from the m group and increase the n group to the same extent; it must be possible for her laying to be represented as m - 1, m - 2, m - 3, etc. females and by n + 1, n + 2, n + 3, etc. males, the sum of m + n remaining constant, but one of the sexes being partly permuted into the other. The ultimate conclusion even cannot be disregarded: we must admit a set of eggs represented by m - m, or zero, females and of n + m males, one of the sexes being completely replaced by the other. Conversely, it must be possible for the feminine series to be augmented from the masculine series to the extent of absorbing it entirely. It was to solve this question and some others connected with it that I undertook, for the second time, to rear the Three-horned Osmia in my study. The problem on this occasion is a more delicate one; but I am also better-equipped. My apparatus consists of two small closed packing-cases, with the front side of each pierced with forty holes, in which I can insert my glass tubes and keep them in a horizontal position. I thus obtain for the Bees the darkness and mystery which suit their work and for myself the power of withdrawing from my hive, at any time, any tube that I wish, with the Osmia inside, so as to carry it to the light and follow, if need be with the aid of the lens, the operations of the busy worker. My investigations, however frequent and minute, in no way hinder the peaceable Bee, who remains absorbed in her maternal duties. I
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