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olume of any weight of water at a known temperature can be readily calculated. Pipettes which are to be used as measuring instruments should also have the relation one to another of the volumes of liquid which they deliver determined, and also the proportions these bear to the values found for the divisions of the burettes in conjunction with which they will be employed. =To Calibrate Tubes for Measuring Gases.=--Prepare a small glass tube sealed at one end and ground at the other to a plate of glass. The tube should hold about as much mercury as will fill 10 mm. divisions of the graduated tube. Fill this tube with mercury, removing all bubbles of air that adhere to the sides by closing the open end of the tube with the thumb, and washing them away with a large air-bubble left for the purpose. If any persistently remain, remove them by means of a fine piece of bone or wood. Then completely fill the tube with mercury, removing any bubbles that may be introduced in the operation, and remove the excess of mercury by placing the ground-glass plate on the mouth of the tube, and pressing it so as to force out all excess of mercury between the two surfaces. Clean the outside of the tube, and place it on a small stand (this may be a small wide-mouthed glass bottle), with which it has been previously weighed when empty, and re-weigh. Repeat this operation several times. From the mean of the results, which should differ one from another but very slightly, the capacity of the tube can be calculated. The purest mercury obtainable should be used. Since the density of pure mercury at 0 deg. C. is 13.596, the weight of mercury required to fill the tube at 0 deg. C., taken in grams, when divided by 13.596, will give the capacity of the tube at 0 deg. C. in cubic centimetres. If the experiment be not made at 0 deg. C., and if a very exact determination of the capacity of the tube be required, the density of mercury must be corrected for expansion or contraction. Having now a vessel of known capacity, it can be employed for ascertaining the capacities of the divisions of a graduated tube in the following manner:--The graduated tube is fixed perpendicularly, mouth upwards, in a secure position. The small tube of known capacity is filled with mercury as previously described, and its contents are transferred to the divided tube. The number of divisions which the known volume of mercury occupies is noted after all air-bubbles have
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